Josephine Elizabeth Siregar

Research Fellow

Qualification:

  • PhD - University of Tokyo, Tokyo - Japan
  • MSc - University of Tokyo, Tokyo - Japan
  • Pgdipl.Sc - University of Queensland, Brisbane - Australia
  • Bsc(Hons) - Universitas Padjadjaran, Bandung - Indonesia

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Interests:

  • I have been interested in the relationship between the mitochondrial function and infectious disease, in particular the potential of mitochondria of the malaria parasite as drug target. The malaria parasite has a complex life cycle involving sudden changes of environment during transmission between different hosts and transition from extra- to intracellular existences, requiring specific adaptive differentiation. Despite the obvious importance of the energy transducing machinery in such adaptive process – which involves aerobic, semi aerobic and relatively anaerobic environment –remarkably little is known about the biogenesis of the mitochondrial energy transducing membrane in the malaria parasite. By acquiring detail knowledge of the biogenesis and function of the parasite’s energy transducing membrane, and particularly the role of the parasite’s two extrachromosomal DNAs in this process, it is hoped that new therapeutic targets for malaria treatment and prophylaxis could be identified.

Publications

  • Within-Host Selection of Drug Resistance in a Mouse Model Reveals Dose-Dependent Selection of Atovaquone Resistance Mutations. Antimicrob Agents Chemother. 2017 Apr 24;61(5). pii: e01867-16. doi: 10.1128/AAC.01867-16. Print 2017 May.
  • Within-Host Selection of Drug Resistance in a Mouse Model of Repeated Incomplete Malaria Treatment: Comparison between Atovaquone and Pyrimethamine. Antimicrob Agents Chemother. 2015 Oct 26;60(1):258-63. doi: 10.1128/AAC.00538-15.
  • Non-invasive surveillance for Plasmodium in reservoir macaque species. Malar J. 2015 Oct 12;14:404. doi: 10.1186/s12936-015-0857-2.
  • Direct evidence for the atovaquone action on the Plasmodium cytochrome bc complex. Parasitol Int. 2014 Sep 28. pii: S1383-5769(14)00133-0. doi: 10.1016/j.parint.2014.09.011.
  • Mutation underlying resistance of Plasmodium berghei to atovaquone in the quinone binding domain 2 (Qo(2)) of the cytochrome b gene. Parasitol Int. 2008 Jun;57(2):229-32. doi: 10.1016/j.parint.2007.12.002. Epub 2007 Dec 8.
  • Molecular basis of antimalarial drug resistance in Indonesia. Adv Exp Med Biol. 2003;531:103-15.
  • Mutations in the cytochrome b gene of Plasmodium berghei conferring resistance to atovaquone. Mol Biochem Parasitol. 1999 Nov 30;104(2):185-94.