To support our research activities, our lab employs virology, immunology, molecular and cell biology techniques.
Dengue Virus Diagnosis dan Detection
Common techniques in dengue detection are used, both for diagnosis in patients' samples and for research purposes:
Dengue Genome Research
We routinely perform dengue genome sequencing for virus genotyping and genomic analysis. Whole genome/gene sequencing are performed using both capillary and the Next Generation Sequencing in our DNA sequencing facility. Published gene sequences have been deposited in GenBank database.
Host Immune Response Study
In our laboratory, we study the host's immune response against dengue infection by looking at the expression profiles of immuno-proteins such as cytokines/chemokines. The activity is performed using Luminex™-based multi-analytes profiling analysis. In addition to the protein expression profiling, the gene expression profiling is also performed using real-time quantitative RT-PCR.
For host's antibody response against dengue infection, we routinely measure the anti-dengue antibody titer using standardized and quality-controlled Plaque Reduction Neutralization Test (PRNT). This method is used in e.g. monitoring the efficacy of dengue vaccine candidate in clinical trial and in dengue sero-prevalence studies.