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Sept. 16, 2019

Virology Seminar and Workshop on Strategies for Arboviral Detection: from Standard to Advance Assays

The seminar & workshop is collaboration between Emerging Virus Research Unit (EVRU) & National Genome Centre (Pusat Genom Nasional) of Eijkman Institute, the United States Centers for Disease Control and Prevention (US CDC) Fort Collins, Colorado, and National University of Singapore (NUS). The three-day workshop from 16th-18th September 2019 with a one-day seminar on 16th September 2019 was held at Eijkman Institute. Seminar and workshop involved intensive lecture and practical as well as discussion to address several strategies and challenges in detecting arbovirus. 

Deputy Director in Translational Research and Hepatitis Unit group leader of Eijkman Institute, Prof. David Handojo Muljono, MD., Sp.PD-FINASIM, Ph.D., formally opened the seminar and welcomed participants. The seminar was attended by 114 participants from various academic institutions, local government units, research and development institutions. Ann M. Powers, Ph.D., Chief of Arbovirology US CDC Fort Collins, gave a lecture on Human Diagnostics for Vector-Borne Diseases. She pointed out about techniques to diagnose vector-borne diseases, laid out their key advantages and limitations. Her presentation was followed by the presentation of Bachti Alisjabana, MD., Sp.PD-KPTI, Ph.D., from Faculty of Medicine, Universitas Padjajaran, who discussed the result of acute febrile illness requiring hospitalization (AFIRE) study by INA-RESPOND. The talk about Multiplex RT-PCR for the simultaneous detection of mosquito-borne viruses was given by Associate Professor of Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, NUS, Justin Jang Hann Chu, Ph.D. He shared method to simultaneously detect, quantify, and differentiate Dengue virus (DENV) and Chikungunya virus (CHIKV) infection with DENV serotyping capability. Frilasita Aisyah Yudhaputri, MBiomedSc. delivered about Central Nervous System Infection in Indonesia on behalf of the head of EVRU, Khin Saw Myint, MD., Ph.D. 

Laboratory training with hands-on learning started in the afternoon of the first day. A total of 25 participants were selected for the workshop. Justin Jang Hann Chu and Chen Huixin of NUS facilitated the first day of workshop. Prior to the practical hands-on session, there was a brief presentation of method on arbovirus multiplex real-time PCR. 

The second day of workshop stressed on analysis of multiplex real-time PCR. This session explained how to interpret multiplex real-time PCR results. Session two of workshop focused on Plaque Reduction Neutralization Test (PRNT). Participants taught how to measure and differentiate Dengue and Zika neutralizing antibody. 

The last day of workshop began with an introduction on Next Generation Sequencing (NGS) as a tool for virus discovery by Jeremy Ledermann, M.S. of US CDC. He discussed the current platforms of NGS. Detailed explanations and tips were given to participants on sequencing RNA viral. The participants also attended the practical hands-on session and equipped with skill on clean up library preparation for NGS in National Genome Centre of the Eijkman Institute. A demo about quality control of library preparation using 4200 TapeStation also given. 

The hands-on session was followed by presentation of Hidayat Trimarsanto from Bioinformatics Unit of Eijkman Institute. He highlighted the use of bioinformatics as an increasingly important tool in the NGS research. Chairman of Eijkman Institute, Prof. Amin Soebandrio, MD., Ph.D., Sp.MK(K), gave token of appreciation to representative participants, Muhammad Fajri Rokhmad from Balai Penelitian dan Pengembangan Kesehatan Papua and Muhammad Yusuf from Hasanuddin University Medical Research Center (HUMRC) Makassar. Finally, Prof. Amin Soebandrio, MD., Ph.D., Sp.MK(K) formally closed the workshop. 

More pictures available on: http://bit.ly/2mm8feB.



The organizing committee would like to thank all the participants for their enthusiasm and hard work as well as the teaching team.

 

Seminar and workshop materials are available here.